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Titre : | Single-Cell Protein Analysis : Methods and Protocols |
Auteurs : | Aik T. Ooi, Auteur |
Type de document : | texte imprimé |
Editeur : | [S.l.] : Humana Press, 2022 |
Collection : | Methodd in Molecular Biology, ISSN 10643745 |
ISBN/ISSN/EAN : | 978-1-07-161773-1 |
Format : | 1 vol.(311 p.) / ill / 24 cm |
Langues: | Anglais |
Index. décimale : | 572 (Biochimie) |
Catégories : |
Biochimie - Biotechnologie - Génie chimique - Génétique ... |
Mots-clés: | Single-cell read outs ; Protein expression data ; Translational research ; Signal amplification |
Résumé : |
This Detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage of new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ranging from simple to complex, conventional to the most current technologies, these chapters offer readers the ability to choose the best suited methodologies for them, based on the sample type and the available technologies or equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Protein Analysis: Methods and Protocols aims to help researchers utilizing single-cell protein analysis in their studies as well to inspire the development of the next generation of improved protein analysis methods in single cells. |
Note de contenu : |
Sommaire : -1- Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections -2- Indirect Immunofluorescence of Tissue Sections -3- Flow Cytometry for Beginners: Hints and Tips for Approaching the Very First Single-Cell Technique -4- High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry -5- Detection of Cytokine-Secreting Cells by Enzyme-Linked Immunospot (ELISpot) -6- Detection and Enumeration of Cytokine-Secreting Cells by FluoroSpot -7- Single-Cell Protein Profiling by Microdroplet Barcoding and Next-Generation Sequencing -8- Single Cell Proteomics Using Multiplexed Isobaric Labeling for Mass Spectrometric Analysis -9- Customizing Maxpar Direct Immune Profiling Assay with Additional -10-High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging -11-Ultra-Sensitive Quantification of Protein and mRNA in Single Mammalian Cells with Digital PLA |
Exemplaires
Code-barres | Cote | Support | Localisation | Section | Disponibilité |
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FB/15584 | SNV8/1787 | Livre | Bibliothèque SNV | Englais | Disponible |