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| Titre : | Assessment of some biological activities of the aqueous and ethanolic extract of the aerial part of Senecio perralderianus and Senecio giganteus | | Type de document : | document électronique | | Auteurs : | Soulaf Kolli, Auteur ; Toufik Krim, Auteur ; Walid Mamache, Directeur de thèse | | Editeur : | Faculté des sciences de la nature et de la vie | | Année de publication : | 2023 | | Importance : | 28 Feuilles | | Format : | 30 cm. | | ISBN/ISSN/EAN : | MS/1585 | | Langues : | Français | | Catégories : | Thèse et mémoire:Master:Biochimie appliquée
| | Mots-clés : | Senecio perralderianus Senecio giganteus Anti-inflammatory Analgesic Gastric
ulcer AAPH Anti-fungal Flavonoids Toxicity. | | Résumé : |
Senecio perralderianus and Senecio giganteus are plants belonging to the Asteraceae family,
obtained from Babor (Sétif) and Maghres mountain. The aerial parts were used to prepare
decoction and ethanolic macerations (Sp Aq, Sp Eth, SG Aq, and Sg Eth). The daily observation
of mice, following oral administration of 2000 or 5000 mg/kg of each extract, showed no signs
of neurological or physical toxicity. The anti-inflammatory properties of the two plant extracts
were evaluated using croton oil-induced ear edema. Sg Aq and Eth extracts showed inhibition
comparable to indomethacin, with inhibition values of 52.25% and 70.63%, respectively.
However, the Sp extracts didn’t exhibit any activity. The analgesic effect induced by acetic acid
(1%) was observed with Sp Eth extract (100 mg/kg), which showed a significant decrease to
19.17 ± 8.78 contractions. Oral administration of HCl-Eth to mice resulted in gastric ulcer
formation, all extracts at a dose of 200 mg/kg showed significant protection against gastric
ulcers, ranging from 68.38% to 75.78%. Pretreatment of mice with the extracts significantly
increased protein content and GSH levels, with a slight increase in SOD and CAT levels, while
reducing in lipid peroxidation. In vitro, the anti-hemolytic activity of Sp aq and Eth extracts at a
concentration of 25 μg/ml effectively protected erythrocytes against AAPH-induced hemolysis,
with values of 150.9 ± 4.686 min and 143.9 ± 1.39 min, respectively. For the anti-fungal activity
all plants extract showed a potent activity against all the fungal strains tested, except for the Sg
Aq extract, which did not show any effect against Fusarium culuorum. The Sg Eth extract
exhibited an effect similar to the control group. On the other hand, the Sp Eth extract showed the
highest effect against all the fungal strains tested. These activities may be attributed to the
presence of flavonoids, phenols compounds in these extracts. |
Assessment of some biological activities of the aqueous and ethanolic extract of the aerial part of Senecio perralderianus and Senecio giganteus [document électronique] / Soulaf Kolli, Auteur ; Toufik Krim, Auteur ; Walid Mamache, Directeur de thèse . - Setif, Algerie : Faculté des sciences de la nature et de la vie, 2023 . - 28 Feuilles ; 30 cm. ISSN : MS/1585 Langues : Français | Catégories : | Thèse et mémoire:Master:Biochimie appliquée
| | Mots-clés : | Senecio perralderianus Senecio giganteus Anti-inflammatory Analgesic Gastric
ulcer AAPH Anti-fungal Flavonoids Toxicity. | | Résumé : |
Senecio perralderianus and Senecio giganteus are plants belonging to the Asteraceae family,
obtained from Babor (Sétif) and Maghres mountain. The aerial parts were used to prepare
decoction and ethanolic macerations (Sp Aq, Sp Eth, SG Aq, and Sg Eth). The daily observation
of mice, following oral administration of 2000 or 5000 mg/kg of each extract, showed no signs
of neurological or physical toxicity. The anti-inflammatory properties of the two plant extracts
were evaluated using croton oil-induced ear edema. Sg Aq and Eth extracts showed inhibition
comparable to indomethacin, with inhibition values of 52.25% and 70.63%, respectively.
However, the Sp extracts didn’t exhibit any activity. The analgesic effect induced by acetic acid
(1%) was observed with Sp Eth extract (100 mg/kg), which showed a significant decrease to
19.17 ± 8.78 contractions. Oral administration of HCl-Eth to mice resulted in gastric ulcer
formation, all extracts at a dose of 200 mg/kg showed significant protection against gastric
ulcers, ranging from 68.38% to 75.78%. Pretreatment of mice with the extracts significantly
increased protein content and GSH levels, with a slight increase in SOD and CAT levels, while
reducing in lipid peroxidation. In vitro, the anti-hemolytic activity of Sp aq and Eth extracts at a
concentration of 25 μg/ml effectively protected erythrocytes against AAPH-induced hemolysis,
with values of 150.9 ± 4.686 min and 143.9 ± 1.39 min, respectively. For the anti-fungal activity
all plants extract showed a potent activity against all the fungal strains tested, except for the Sg
Aq extract, which did not show any effect against Fusarium culuorum. The Sg Eth extract
exhibited an effect similar to the control group. On the other hand, the Sp Eth extract showed the
highest effect against all the fungal strains tested. These activities may be attributed to the
presence of flavonoids, phenols compounds in these extracts. |
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Affiner la rechercheAssessment of some biological activities of the aqueous and ethanolic extract of the aerial part of Senecio perralderianus and Senecio giganteus / Soulaf Kolli / Faculté des sciences de la nature et de la vie (2023)
