Affinity chromatography / [Totowa (N.J.)] : Humana press (2008)

Public ISBD Titre : Affinity chromatography : methods and protocols Type de document : texte imprimé Auteurs : Michael Zachariou, Editeur scientifique Mention d'édition : Second ed. Editeur : [Totowa (N.J.)] : Humana press Année de publication : 2008 Collection : Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745 num. 421 Importance : 1 vol. (XII-343 p.) Présentation : ill. Format : 24 cm ISBN/ISSN/EAN : 978-1-59745-582-4 Langues : Anglais Catégories : Biochimie - Biotechnologie - Génie chimique - Génétique ... Chimie ( Organique -Générale- Théorique - Analytique -Quantique - Géochimie - Physique ) Mots-clés : - Chromatographie - Chromatographie d'affinité Index. décimale : 543.8 Chromatographie Résumé : This chapter covers the use of immobilized metal ion affinity chromatography (IMAC) for enrichment of phosphorylated proteins. Some requirements for successful enrichment of these types of proteins are discussed. An experimental protocol and a set of application data are included to enable the scientist to obtain high-yield results in a very short time with pre-packed phospho-specific metal ion affinity resin (PMAC). Note de contenu : Sommaire: Roque, Ana Cecília A. (et al.)Pages 1-23 Part 1: Various modes of affinity Chromatography Immobilized Metal Ion Affinity Chromatography of Native Proteins Charlton, Adam (et al.Pages 25-36 Affinity Precipitation of Proteins Using Metal Chelates Kumar, Ashok (et al.)Pages 37-52 Immunoaffinity Chromatography Gallant, Stuart R. (et al.)Pages 53-60 Dye Ligand Chromatography allant, Stuart R. (et al.)Pages 61-70 Purification of Proteins Using Displacement Chromatography Tugcu, NihalPages 71-90 Part 2:Affinity Chromatography using Rationally Designed Ligands for Use in Affinity Chromatography Roque, Ana Cecília A. (et al.)Pages 93-110 Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography Casey, Joanne L. (et al.)Pages 111-124 Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein Forde, Gareth M.Pages 125-136 Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins Charlton, Adam (et al.)Pages 137-150 Methods for the Purification of HQ-Tagged Proteins Godat, Becky (et al.)Pages 151-168 Amylose Affinity Chromatography of Maltose-Binding Protein Pattenden, Leonard K (et al.)Pages 169-190 Methods for Detection of Protein–Proteinnl and Protein–DNA Interactions Using HaloTag ™ Urh, Marjeta (et al.)Pages 191-210 Site-Specific Cleavage of Fusion Proteins Charlton, Adam Pages 211-228 The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags Arnau, José (et al.)Pages 229-243 Part 3:Various applications of Affinity Chromatography Affinity Processing of Cell-Containing Feeds Using monolithic Macroporous Hydrogels, Cryogels Galaev, Igor Yu. (et al.)Pages 247-256 Monolithic Bioreactors for Macromolecules Benčina, Mojca (et al.)Pages 257-274 Plasmid DNA Purification Via the Use of a Dual Affinity Protein Forde, Gareth M.Pages 275-284 Affinity Chromatography of Phosphorylated Proteins Tchaga, Grigoriy S.Pages 285-294 Protein Separation Using Immobilized Phospholipid Chromatography Lee, Tzong-Hsien (et al.)Pages 295-302 Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis Heegaard, Niels H. H. (et al.) Affinity chromatography : methods and protocols [texte imprimé] / Michael Zachariou, Editeur scientifique  . -  Second ed. . - [Totowa (N.J.)] : Humana press, 2008 . - 1 vol. (XII-343 p.) : ill. ; 24 cm. - (Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745; 421) . ISBN : 978-1-59745-582-4 Langues : Anglais Catégories : Biochimie - Biotechnologie - Génie chimique - Génétique ... Chimie ( Organique -Générale- Théorique - Analytique -Quantique - Géochimie - Physique ) Mots-clés : - Chromatographie - Chromatographie d'affinité Index. décimale : 543.8 Chromatographie Résumé : This chapter covers the use of immobilized metal ion affinity chromatography (IMAC) for enrichment of phosphorylated proteins. Some requirements for successful enrichment of these types of proteins are discussed. An experimental protocol and a set of application data are included to enable the scientist to obtain high-yield results in a very short time with pre-packed phospho-specific metal ion affinity resin (PMAC). Note de contenu : Sommaire: Roque, Ana Cecília A. (et al.)Pages 1-23 Part 1: Various modes of affinity Chromatography Immobilized Metal Ion Affinity Chromatography of Native Proteins Charlton, Adam (et al.Pages 25-36 Affinity Precipitation of Proteins Using Metal Chelates Kumar, Ashok (et al.)Pages 37-52 Immunoaffinity Chromatography Gallant, Stuart R. (et al.)Pages 53-60 Dye Ligand Chromatography allant, Stuart R. (et al.)Pages 61-70 Purification of Proteins Using Displacement Chromatography Tugcu, NihalPages 71-90 Part 2:Affinity Chromatography using Rationally Designed Ligands for Use in Affinity Chromatography Roque, Ana Cecília A. (et al.)Pages 93-110 Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography Casey, Joanne L. (et al.)Pages 111-124 Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein Forde, Gareth M.Pages 125-136 Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins Charlton, Adam (et al.)Pages 137-150 Methods for the Purification of HQ-Tagged Proteins Godat, Becky (et al.)Pages 151-168 Amylose Affinity Chromatography of Maltose-Binding Protein Pattenden, Leonard K (et al.)Pages 169-190 Methods for Detection of Protein–Proteinnl and Protein–DNA Interactions Using HaloTag ™ Urh, Marjeta (et al.)Pages 191-210 Site-Specific Cleavage of Fusion Proteins Charlton, Adam Pages 211-228 The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags Arnau, José (et al.)Pages 229-243 Part 3:Various applications of Affinity Chromatography Affinity Processing of Cell-Containing Feeds Using monolithic Macroporous Hydrogels, Cryogels Galaev, Igor Yu. (et al.)Pages 247-256 Monolithic Bioreactors for Macromolecules Benčina, Mojca (et al.)Pages 257-274 Plasmid DNA Purification Via the Use of a Dual Affinity Protein Forde, Gareth M.Pages 275-284 Affinity Chromatography of Phosphorylated Proteins Tchaga, Grigoriy S.Pages 285-294 Protein Separation Using Immobilized Phospholipid Chromatography Lee, Tzong-Hsien (et al.)Pages 295-302 Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis Heegaard, Niels H. H. (et al.)

Exemplaires

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FB/06653	SNV8/0933	Livre	Bibliothèque SNV	Englais	Disponible
FB/06654	SNV8/0933	Livre	Bibliothèque SNV	Englais	Disponible
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FB/06680	SNV8/0933	Livre	Bibliothèque SNV	Englais	Disponible
FB/06681	SNV8/0933	Livre	Bibliothèque SNV	Englais	Disponible

Bioreactors in Stem Cell Biology / Kursad Turksen / [Totowa (N.J.)] : Humana press (2022) 

Public ISBD Titre : Bioreactors in Stem Cell Biology : Methods and Protocols Type de document : texte imprimé Auteurs : Kursad Turksen, Auteur Editeur : [Totowa (N.J.)] : Humana press Année de publication : 2022 Collection : Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745 Importance : 1 vol. (285 p.) Présentation : ill. en coul. Format : 24 cm ISBN/ISSN/EAN : 978-1-07-162020-5 Langues : Français Catégories : Biologie ( B-Cellulaire - B-Animale - B-Végétale - B-moléculaire - Cytologie ) Mots-clés : Mesenchymal stem cells  Human pluripotent stem cells  Cell culture  Pharmaceuticals Index. décimale : 571.6 Biologie cellulaire Résumé : This New edition brings together some of the latest developments and protocols reflecting the rapidity with which bioreactor technologies are advancing and being applied. Given that the use of bioreactors in cell biology is becoming more commonplace as attempts are made to scale-up production of various types of cells for regenerative medicine and pharmaceutical purposes, this volume provides practical guidance for navigating research projects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bioreactors in Stem Cell Biology: Methods and Protocols, Second Edition will benefit both established investigators and newcomers to this dynamic area of study. Note de contenu : Sommaire: - Benchtop Bioreactors in Mammalian Cell Culture: Overview and Guidelines - Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor - Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter Volume Bioreactors for Cell Culturing - Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors - High-Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors - Integrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture - Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor - Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor - Optimized Method to Improve Cell Activity in 3D Scaffolds Under a Dual Real-Time Dynamic Bioreactor System - In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor - Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor - Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor Bioreactors in Stem Cell Biology : Methods and Protocols [texte imprimé] / Kursad Turksen, Auteur . - [Totowa (N.J.)] : Humana press, 2022 . - 1 vol. (285 p.) : ill. en coul. ; 24 cm. - (Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745) . ISBN : 978-1-07-162020-5 Langues : Français Catégories : Biologie ( B-Cellulaire - B-Animale - B-Végétale - B-moléculaire - Cytologie ) Mots-clés : Mesenchymal stem cells  Human pluripotent stem cells  Cell culture  Pharmaceuticals Index. décimale : 571.6 Biologie cellulaire Résumé : This New edition brings together some of the latest developments and protocols reflecting the rapidity with which bioreactor technologies are advancing and being applied. Given that the use of bioreactors in cell biology is becoming more commonplace as attempts are made to scale-up production of various types of cells for regenerative medicine and pharmaceutical purposes, this volume provides practical guidance for navigating research projects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bioreactors in Stem Cell Biology: Methods and Protocols, Second Edition will benefit both established investigators and newcomers to this dynamic area of study. Note de contenu : Sommaire: - Benchtop Bioreactors in Mammalian Cell Culture: Overview and Guidelines - Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor - Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter Volume Bioreactors for Cell Culturing - Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors - High-Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors - Integrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture - Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor - Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor - Optimized Method to Improve Cell Activity in 3D Scaffolds Under a Dual Real-Time Dynamic Bioreactor System - In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor - Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor - Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor

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FB/15500	SNV8/1745	Livre	Bibliothèque SNV	Englais	Disponible
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Glycosylation / Gavin P. Davey / [Totowa (N.J.)] : Humana press (2022) 

Public ISBD Titre : Glycosylation : Methods and Protocols Type de document : texte imprimé Auteurs : Gavin P. Davey, Auteur Editeur : [Totowa (N.J.)] : Humana press Année de publication : 2022 Collection : Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745 Importance : 1 vol. (332 p.) Présentation : ill. en coul. Format : 24 cm ISBN/ISSN/EAN : 978-1-07-161687-1 Langues : Français Catégories : Bio-informatique - Biostatistiques - Biomathématique - Mathématique - Informatique Mots-clés : biotherapeutics development  glycoengineering  Click-chemistry  STED microscopy  Epigenetic control Index. décimale : 616.079 immunochimie Résumé : This Volume explores the latest techniques and methods used for performing up-to-date glycosylation research. The chapters in this book are organized into four parts. Part One looks at the latest analytical and bioinformatics technologies that enable the characterization of glycosylation complexity. Part Two details the importance of synthetic chemistry and glycoengineering in the fields of bioprocessing and biotherapeutic development. Part Three discusses systems biology and computational technologies used by scientists to analyze glycosylation events in the cell. Part Four focuses on how cellular glycosylation biomarkers can be identified and used to characterize human clinical datasets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Note de contenu : Sommer: * Part 1 Analytical and Bioinformatics Methods for Studying Glycosylation * Part 2 Synthetic Biology and Glycoengineering Methods for Controlling Glycosylation * Part 3 Systems Biology and Computational Methods for Deciphering Glycosylation Networks * Part 4 Glycosylation Biomarkers and Clinical Datasets Glycosylation : Methods and Protocols [texte imprimé] / Gavin P. Davey, Auteur . - [Totowa (N.J.)] : Humana press, 2022 . - 1 vol. (332 p.) : ill. en coul. ; 24 cm. - (Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745) . ISBN : 978-1-07-161687-1 Langues : Français Catégories : Bio-informatique - Biostatistiques - Biomathématique - Mathématique - Informatique Mots-clés : biotherapeutics development  glycoengineering  Click-chemistry  STED microscopy  Epigenetic control Index. décimale : 616.079 immunochimie Résumé : This Volume explores the latest techniques and methods used for performing up-to-date glycosylation research. The chapters in this book are organized into four parts. Part One looks at the latest analytical and bioinformatics technologies that enable the characterization of glycosylation complexity. Part Two details the importance of synthetic chemistry and glycoengineering in the fields of bioprocessing and biotherapeutic development. Part Three discusses systems biology and computational technologies used by scientists to analyze glycosylation events in the cell. Part Four focuses on how cellular glycosylation biomarkers can be identified and used to characterize human clinical datasets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Note de contenu : Sommer: * Part 1 Analytical and Bioinformatics Methods for Studying Glycosylation * Part 2 Synthetic Biology and Glycoengineering Methods for Controlling Glycosylation * Part 3 Systems Biology and Computational Methods for Deciphering Glycosylation Networks * Part 4 Glycosylation Biomarkers and Clinical Datasets

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FB/15547	SNV8/1769	Livre	Bibliothèque SNV	Englais	Disponible
FB/15546	SNV8/1769	Livre	Bibliothèque SNV	Englais	Disponible

Plant cell division / Marie-Cécile Caillaud / [Totowa (N.J.)] : Humana press (2022)

Public ISBD Titre : Plant cell division : Methods and Protocols Type de document : texte imprimé Auteurs : Marie-Cécile Caillaud Editeur : [Totowa (N.J.)] : Humana press Année de publication : 2022 Collection : Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745 Importance : 1 vol. (267 p.) Présentation : ill. en coul. Format : 24 cm ISBN/ISSN/EAN : 978-1-07-161746-5 Langues : Anglais Langues originales : Anglais Catégories : Biologie ( B-Cellulaire - B-Animale - B-Végétale - B-moléculaire - Cytologie ) Mots-clés : - Cell cycle progression - Mitosis - Cell division patterning - Cytokinesis - Progression du cycle cellulaire - Mitose - Organisation de la division cellulaire - Cytokinèse Index. décimale : 581 Biologie végétale Résumé : This New edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression. Note de contenu : Sommaire: * Part I: Mitosis and Cell Cycle Progression * Part II: Manipulation of Cell Division * Part III: Quantification of Cell Division Patterning * Part IV: Imaging and Quantifying Plant Cytokinesis Plant cell division : Methods and Protocols [texte imprimé] / Marie-Cécile Caillaud . - [Totowa (N.J.)] : Humana press, 2022 . - 1 vol. (267 p.) : ill. en coul. ; 24 cm. - (Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745) . ISBN : 978-1-07-161746-5 Langues : Anglais Langues originales : Anglais Catégories : Biologie ( B-Cellulaire - B-Animale - B-Végétale - B-moléculaire - Cytologie ) Mots-clés : - Cell cycle progression - Mitosis - Cell division patterning - Cytokinesis - Progression du cycle cellulaire - Mitose - Organisation de la division cellulaire - Cytokinèse Index. décimale : 581 Biologie végétale Résumé : This New edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression. Note de contenu : Sommaire: * Part I: Mitosis and Cell Cycle Progression * Part II: Manipulation of Cell Division * Part III: Quantification of Cell Division Patterning * Part IV: Imaging and Quantifying Plant Cytokinesis

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