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| Titre : | Evaluation of anti-inflammatory and antioxidant activity of Ziziphus spina L leaves extracts | | Type de document : | document électronique | | Auteurs : | Isra Cheber, Auteur ; Aya Charfaoui, Auteur ; Seddik Khennouf, Directeur de thèse | | Editeur : | Sétif : Faculté des Sciences de la Nature et de la Vie | | Année de publication : | 2024 | | Importance : | 45 Feuilles | | ISBN/ISSN/EAN : | MS/2042 | | Langues : | Anglais | | Catégories : | Thèse et mémoire:Master:Physiologie cellulaire et physiopathologie
| | Mots-clés : | Ziziphus spina christi Anti-inflammatory activity Antioxidant activity Polyphenols Flavonoids. | | Résumé : |
This study aims to evaluate the anti-inflammatory and antioxidant activity of ethanolic (Eth.E), hydro-ethanolic (HEE) and aqueous (Aq.E) extracts of Ziziphus spina christi leaves. The quantitive assessment of phenolic compounds showed that Aq.E extract of this plant contains 634.155 ug GAE/mg for total polyphenols , 4.451 ug QE/mg for flavonoids and 869.77 ug CE/ mg for tannins and total polyphenolic content of ethanolic extract was 496.911 ug GAE/mg ,8.718 ug QE/g for flavonoids content and 377.6 μg CE/mg compared to hydro-ethanolic extract 409.244 ug GAE/mg for polyphenols, 6.730 ug QE/mg for flavonoids and 884.66 ug CE/mg. The antioxidant activity of Ethanolic , hydro-ethanolic and aqueous extracts was estimated in vitro by determining the activity of DPPH (2’2-diphenylpicrylhydrazyl) ,ferrous ion chelation (FIC) and peroxidase (H2O2) .The results showed that Eth .E, Aq.E and hydro-ethanolic extracts inhibited DPPH activity with IC50 values of 0.031±0.006mg/mL ,0.066±0.005 mg/mL and 0.037±0.002 mg/mL ,respectively and they chelated ferrous ion with IC50 0.082±0.04mg/mL , 0.06±0.00mg/mL and 0.23±0.00mg/mL respectively . The ethanolic ,hydro-ethanolic and aqueous extracts also showed an ability to eliminate hydrogen peroxide with IC50 values of 0.029 ±0.001mg/mL,0.037±0.001mg/mL and 0.021±0.005 mg/mL, respectively. The result of anti-inflammatory activity in vitro demonstrated that the hydro-ethanolic extract showed an ability to inhibit the egg albumin denaturation with value of 92.49 ±2.21% compared with control 57.16 ±45.63%. Moreover In vivo the hydro-ethanolic extract reduced the thickness of xylene-stimulated oedema at concentration of 200mg/kg and 400mg/kg with value of 0.074± 0.04mm and 0.09± 0.05 mm respectively compared to the control 0.39± 0.12 mm. The result of inflammation induced with xylene compared to voltaren showed that the hydro-ethanolic extract reduced the inflammation with 64.64% . |
Evaluation of anti-inflammatory and antioxidant activity of Ziziphus spina L leaves extracts [document électronique] / Isra Cheber, Auteur ; Aya Charfaoui, Auteur ; Seddik Khennouf, Directeur de thèse . - Sétif : Faculté des Sciences de la Nature et de la Vie, 2024 . - 45 Feuilles. ISSN : MS/2042 Langues : Anglais | Catégories : | Thèse et mémoire:Master:Physiologie cellulaire et physiopathologie
| | Mots-clés : | Ziziphus spina christi Anti-inflammatory activity Antioxidant activity Polyphenols Flavonoids. | | Résumé : |
This study aims to evaluate the anti-inflammatory and antioxidant activity of ethanolic (Eth.E), hydro-ethanolic (HEE) and aqueous (Aq.E) extracts of Ziziphus spina christi leaves. The quantitive assessment of phenolic compounds showed that Aq.E extract of this plant contains 634.155 ug GAE/mg for total polyphenols , 4.451 ug QE/mg for flavonoids and 869.77 ug CE/ mg for tannins and total polyphenolic content of ethanolic extract was 496.911 ug GAE/mg ,8.718 ug QE/g for flavonoids content and 377.6 μg CE/mg compared to hydro-ethanolic extract 409.244 ug GAE/mg for polyphenols, 6.730 ug QE/mg for flavonoids and 884.66 ug CE/mg. The antioxidant activity of Ethanolic , hydro-ethanolic and aqueous extracts was estimated in vitro by determining the activity of DPPH (2’2-diphenylpicrylhydrazyl) ,ferrous ion chelation (FIC) and peroxidase (H2O2) .The results showed that Eth .E, Aq.E and hydro-ethanolic extracts inhibited DPPH activity with IC50 values of 0.031±0.006mg/mL ,0.066±0.005 mg/mL and 0.037±0.002 mg/mL ,respectively and they chelated ferrous ion with IC50 0.082±0.04mg/mL , 0.06±0.00mg/mL and 0.23±0.00mg/mL respectively . The ethanolic ,hydro-ethanolic and aqueous extracts also showed an ability to eliminate hydrogen peroxide with IC50 values of 0.029 ±0.001mg/mL,0.037±0.001mg/mL and 0.021±0.005 mg/mL, respectively. The result of anti-inflammatory activity in vitro demonstrated that the hydro-ethanolic extract showed an ability to inhibit the egg albumin denaturation with value of 92.49 ±2.21% compared with control 57.16 ±45.63%. Moreover In vivo the hydro-ethanolic extract reduced the thickness of xylene-stimulated oedema at concentration of 200mg/kg and 400mg/kg with value of 0.074± 0.04mm and 0.09± 0.05 mm respectively compared to the control 0.39± 0.12 mm. The result of inflammation induced with xylene compared to voltaren showed that the hydro-ethanolic extract reduced the inflammation with 64.64% . |
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Affiner la rechercheEvaluation of anti-inflammatory and antioxidant activity of Ziziphus spina L leaves extracts / Isra Cheber / Sétif : Faculté des Sciences de la Nature et de la Vie (2024)
