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| Titre : | Production of Exopolysaccharide by Fermentation | | Type de document : | document électronique | | Auteurs : | Zakarya El-Moatazz Menadi, Auteur ; Gheraibia Sara, Directeur de thèse | | Editeur : | Sétif [Algérie] : Faculté des sciences de la Nature et de la vie, Université Ferhat Abbas Sétif 1 | | Année de publication : | 2025 | | Importance : | 67 Feuilles | | Format : | PDF | | ISBN/ISSN/EAN : | MS/2505 | | Langues : | Anglais | | Catégories : | Thèse et mémoire:Master:Biochimie appliquée
| | Mots-clés : | Dextran Microbial fermentation Beet juice Carrot juice Leuconostoc mesenteroides Lactobacillus FTIR. | | Résumé : |
Screening dextran-producing strains and selecting optimal carbon sources are essential steps to maximize dextran yield and obtain structures with functional properties suited for various industrial applications. This study aimed to evaluate the production of dextran through microbial fermentation using a mixed culture of Leuconostoc mesenteroides and Lactobacillus species, with three different carbon sources: pure saccharose, beet juice (9° Brix), and carrot juice (6° Brix). The investigation assessed dextran concentration, conversion yield, pH variation over time, solubility behavior, structural confirmation via FTIR, and a comparative analysis of two dextran recovery methods.The results demonstrated that beet juice was the most effective carbon source, producing the highest dextran concentration (19.0 g/L) and yield (0.21 g/g), surpassing the performance of pure saccharose (15.8 g/L, 0.125 g/g) and carrot juice (~5.8 g/L, ~0.097 g/g). The beet juice medium also showed a slower pH decline, which may have prolonged the activity of dextransucrase during fermentation. While saccharose provided consistent and predictable results, its higher sugar input made its yield relatively less efficient. Carrot juice, due to its low sucrose content and potential presence of inhibitory compounds, was the least effective substrate. dextran can be efficiently produced using non-conventional carbon sources, particularly beet juice, under mixed-culture fermentation, providing a valuable contribution to biopolymer research and green biotechnology. |
Production of Exopolysaccharide by Fermentation [document électronique] / Zakarya El-Moatazz Menadi, Auteur ; Gheraibia Sara, Directeur de thèse . - Sétif (Algérie) : Faculté des sciences de la Nature et de la vie, Université Ferhat Abbas Sétif 1, 2025 . - 67 Feuilles ; PDF. ISSN : MS/2505 Langues : Anglais | Catégories : | Thèse et mémoire:Master:Biochimie appliquée
| | Mots-clés : | Dextran Microbial fermentation Beet juice Carrot juice Leuconostoc mesenteroides Lactobacillus FTIR. | | Résumé : |
Screening dextran-producing strains and selecting optimal carbon sources are essential steps to maximize dextran yield and obtain structures with functional properties suited for various industrial applications. This study aimed to evaluate the production of dextran through microbial fermentation using a mixed culture of Leuconostoc mesenteroides and Lactobacillus species, with three different carbon sources: pure saccharose, beet juice (9° Brix), and carrot juice (6° Brix). The investigation assessed dextran concentration, conversion yield, pH variation over time, solubility behavior, structural confirmation via FTIR, and a comparative analysis of two dextran recovery methods.The results demonstrated that beet juice was the most effective carbon source, producing the highest dextran concentration (19.0 g/L) and yield (0.21 g/g), surpassing the performance of pure saccharose (15.8 g/L, 0.125 g/g) and carrot juice (~5.8 g/L, ~0.097 g/g). The beet juice medium also showed a slower pH decline, which may have prolonged the activity of dextransucrase during fermentation. While saccharose provided consistent and predictable results, its higher sugar input made its yield relatively less efficient. Carrot juice, due to its low sucrose content and potential presence of inhibitory compounds, was the least effective substrate. dextran can be efficiently produced using non-conventional carbon sources, particularly beet juice, under mixed-culture fermentation, providing a valuable contribution to biopolymer research and green biotechnology. |
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Affiner la rechercheProduction of Exopolysaccharide by Fermentation / Zakarya El-Moatazz Menadi / Sétif [Algérie] : Faculté des sciences de la Nature et de la vie, Université Ferhat Abbas Sétif 1 (2025)
