Titre :	In vitro and In vivo anti-inflammatory and hepatoprotector effects of Algerian Echium plantagineum and Brassica rapa extracts
Type de document :	document électronique
Auteurs :	Soumia Benbrinis, Auteur ; Abderrahmane Senator, Directeur de thèse
Année de publication :	2024
Importance :	1 vol (107 f.) / ill.en coul.
Format :	DVD.
ISBN/ISSN/EAN :	DOC-E/279
Langues :	Français
Catégories :	Thèse et mémoire:Doctorat:Département de biochimie
Mots-clés :	Echium plantagineum  Brassica rapa
Résumé :	This study aims to explore the phenolic composition of aqueous(Aq E)and methanolic(Met E)extracts of Algerian Echium plantagineum aerial part and evaluate their antioxidant capacity. Methods:Total phenolics, flavonoids and tannins contents were determined by spectrophotometric methods using Folin-Ciocalteu, aluminium chloride and hemoglobin precipitation assays,respectively.The antioxidant activities were evaluated using several in vitro tests including reactive oxygen species(ABTS.,OH.,O2.and H2O2)scavenging assays,ferrous ions chelating test, reducing power and anti-hemolytic assays.Results: The phytochemical study revealed a predominance of tannins, total polyphenols and flavonoids in both extracts.Aq E showed more efficiency to scavenge ABTS.,O2.and OH. radicals and to chelate ferrous ions. However,Met E was more efficient(IC50=193.87μg/mL)in scavenging H2O2 than Aq E (IC50 = 262.46μg/mL) . In the same way,Met E inhibited AAPH induced hemolysis(HT50=179.79±5.82 min)more than Aq E(HT50=161.62±5.87 min).On the other hand,both Met E and Aq E Exhibited very high reducing power (IC50= 117μg/mL and 128μg/mL, respectively). Conclusion: This study shows that Echium plantagineum extracts possess strong antioxidant activity,which is probably due to their phenolic composition.Therefore, this plant can constitute a promising source of natural antioxidants.
En ligne :	http://dspace.univ-setif.dz:8888/jspui/bitstream/123456789/4292/1/Th%c3%a8se%20B [...]

In vitro and In vivo anti-inflammatory and hepatoprotector effects of Algerian Echium plantagineum and Brassica rapa extracts [document électronique] / Soumia Benbrinis, Auteur ; Abderrahmane Senator, Directeur de thèse . - 2024 . - 1 vol (107 f.) / ill.en coul. ; DVD.
ISSN : DOC-E/279
Langues : Français

Catégories :	Thèse et mémoire:Doctorat:Département de biochimie
Mots-clés :	Echium plantagineum  Brassica rapa
Résumé :	This study aims to explore the phenolic composition of aqueous(Aq E)and methanolic(Met E)extracts of Algerian Echium plantagineum aerial part and evaluate their antioxidant capacity. Methods:Total phenolics, flavonoids and tannins contents were determined by spectrophotometric methods using Folin-Ciocalteu, aluminium chloride and hemoglobin precipitation assays,respectively.The antioxidant activities were evaluated using several in vitro tests including reactive oxygen species(ABTS.,OH.,O2.and H2O2)scavenging assays,ferrous ions chelating test, reducing power and anti-hemolytic assays.Results: The phytochemical study revealed a predominance of tannins, total polyphenols and flavonoids in both extracts.Aq E showed more efficiency to scavenge ABTS.,O2.and OH. radicals and to chelate ferrous ions. However,Met E was more efficient(IC50=193.87μg/mL)in scavenging H2O2 than Aq E (IC50 = 262.46μg/mL) . In the same way,Met E inhibited AAPH induced hemolysis(HT50=179.79±5.82 min)more than Aq E(HT50=161.62±5.87 min).On the other hand,both Met E and Aq E Exhibited very high reducing power (IC50= 117μg/mL and 128μg/mL, respectively). Conclusion: This study shows that Echium plantagineum extracts possess strong antioxidant activity,which is probably due to their phenolic composition.Therefore, this plant can constitute a promising source of natural antioxidants.
En ligne :	http://dspace.univ-setif.dz:8888/jspui/bitstream/123456789/4292/1/Th%c3%a8se%20B [...]