Titre :	Antioxidant and anticoagulant effects of Aqueous extract of Inula viscosa
Type de document :	document électronique
Auteurs :	Besma Lamari, Auteur ; Amira Derafa, Auteur ; Hamama Bouriche, Directeur de thèse
Editeur :	Sétif [Algérie] : Faculté des sciences de la Nature et de la vie, Université Ferhat Abbas Sétif 1
Année de publication :	2024
Importance :	38 Feuilles
Format :	PDF
ISBN/ISSN/EAN :	MS/2001
Langues :	Anglais
Catégories :	Thèse et mémoire:Master:Biochimie appliquée
Mots-clés :	- Antioxidant  Anticoagulant  Flavonoids  Inula viscosa  Polyphenols  Prothrombin time.
Résumé :	The present study aims to evaluate the antioxidant and anticoagulant activities of aqueous extract (Aq. E) of Inula viscosa (I. viscosa) leaf. The total polyphenol and flavonoids content of Aq. E was quantified spectrophotometrically, using Folin-Ciocalteu and AlCl3, respectively. Results indicate that the studied extract contain considerable amounts of polyphenols (129. 93 ± 5.76 μg GAE/ mg) and flavonoids (12.12 ± 5.05 μg QE/mg). The antioxidant activity of I. viscosa leaf was estimated in vitro by determining the scavenging activity of DPPH. and reducing power capacity. Results showed that the Aq. E inhibited DPPH. with IC50 values of 14.77 ± 0.29 μg/ml. Moreover, the Aq.E exhibited powerful reducing power (EC50 = 45.81 ± 0.92 μg/ml). On the other hand, the anticoagulant activity of I. viscosa leaf extract was evaluated the anti-coagulation by recording the time required for complete blood clotting (prothrombin time TP). Results revealed that extract of I.viscosa at 50 μg/ml exerted significant anticoagulant activity, where the clotting time was prolonged to 19:49 min. Moreover, analysis of the effect of I. viscosa extract at different concentrations (1, 10, 20 and 50 μg/ml) on the clot lysis revealed that the Aq. E increased the lysis of human clot. The highest percentage (23.56 ± 12.24) of the clot lysis was obtained at 1 μg/ml. This value was much close to that obtained with citrate (22.63± 3.65 %), used as positive control. The obtained results showed that the extract of Inula viscose possess considerable antioxidant, anticoagulant and antithrombotic effects, which qualified as an important source of bioactive compounds with potential interesting therapeutic applications.
En ligne :	http://dspace.univ-setif.dz:8888/jspui/bitstream/123456789/5680/1/BAPP-CD227---- [...]

Antioxidant and anticoagulant effects of Aqueous extract of Inula viscosa [document électronique] / Besma Lamari, Auteur ; Amira Derafa, Auteur ; Hamama Bouriche, Directeur de thèse . - Sétif (Algérie) : Faculté des sciences de la Nature et de la vie, Université Ferhat Abbas Sétif 1, 2024 . - 38 Feuilles ; PDF.
ISSN : MS/2001
Langues : Anglais

Catégories :	Thèse et mémoire:Master:Biochimie appliquée
Mots-clés :	- Antioxidant  Anticoagulant  Flavonoids  Inula viscosa  Polyphenols  Prothrombin time.
Résumé :	The present study aims to evaluate the antioxidant and anticoagulant activities of aqueous extract (Aq. E) of Inula viscosa (I. viscosa) leaf. The total polyphenol and flavonoids content of Aq. E was quantified spectrophotometrically, using Folin-Ciocalteu and AlCl3, respectively. Results indicate that the studied extract contain considerable amounts of polyphenols (129. 93 ± 5.76 μg GAE/ mg) and flavonoids (12.12 ± 5.05 μg QE/mg). The antioxidant activity of I. viscosa leaf was estimated in vitro by determining the scavenging activity of DPPH. and reducing power capacity. Results showed that the Aq. E inhibited DPPH. with IC50 values of 14.77 ± 0.29 μg/ml. Moreover, the Aq.E exhibited powerful reducing power (EC50 = 45.81 ± 0.92 μg/ml). On the other hand, the anticoagulant activity of I. viscosa leaf extract was evaluated the anti-coagulation by recording the time required for complete blood clotting (prothrombin time TP). Results revealed that extract of I.viscosa at 50 μg/ml exerted significant anticoagulant activity, where the clotting time was prolonged to 19:49 min. Moreover, analysis of the effect of I. viscosa extract at different concentrations (1, 10, 20 and 50 μg/ml) on the clot lysis revealed that the Aq. E increased the lysis of human clot. The highest percentage (23.56 ± 12.24) of the clot lysis was obtained at 1 μg/ml. This value was much close to that obtained with citrate (22.63± 3.65 %), used as positive control. The obtained results showed that the extract of Inula viscose possess considerable antioxidant, anticoagulant and antithrombotic effects, which qualified as an important source of bioactive compounds with potential interesting therapeutic applications.
En ligne :	http://dspace.univ-setif.dz:8888/jspui/bitstream/123456789/5680/1/BAPP-CD227---- [...]