Titre :	Estimation du contenu phenolique et evaluation de l’activite antioxydante de l’extrait hydromethanolique de teucrium flavum l.
Type de document :	document électronique
Auteurs :	Hadil Doumi, Auteur ; Taklit Ferhoune, Auteur ; Hassiba Benabdallah, Directeur de thèse
Editeur :	Sétif : Faculté des Sciences de la Nature et de la Vie
Année de publication :	2024
Importance :	44 Feuilles
ISBN/ISSN/EAN :	MS/2043
Langues :	Français
Catégories :	Thèse et mémoire:Master:Physiologie cellulaire et physiopathologie
Mots-clés :	Teucrium flavum L.  Antioxidant activity  Phenolic compounds  BHT  Phenanthroline.
Résumé :	Teucrium flavum L., known by the common name of "Germandrée jaune", is a medicinal plant of the Lamiaceaes family , widely used in traditional medicine as antipyretic and healing. In this work, a hydromethanolic extract was prepared from the aerial part of this plant. The extraction yield was 24.54%. Quantitative estimation of phenolic compounds by colorimetric method showed that the extract contains 203.00 ± 0.003 mg gallic acid equivalent/g of extract, 12.00 ± 0.001 mg quercetin equivalent/g of extract and 226.00 ± 0.0002 mg tannic acid equivalent/g of extract. The evaluation of antioxidant power was performed using different tests in the presence of standard antioxidants. The results revealed that the extract showed low activity against the DPPH radical with an IC50 value of 0.0184 ± 0.0004 mg/mL compared to the standard antioxidant, gallic acid, (IC50= 0.001 ± 4.11E-05 mg/mL). For the H2O2 test, the IC50 value is 0.363 ± 0.087 mg/mL. The reducing power of the hydromethanolic extract of Teucrium flavum L. (A0.5 = 0.024 ± 0.0001 mg/mL) is lower than that of the standard antioxidant (A0.5 = 0.0143 ± 0.0003 mg/mL). Same thing was observed for the phenanthroline assay where the extract showed low activity (A0.5 = 0.012 ± 0.001 mg/mL) compared to BHT whose A0.5 value was 0.002 ± 7.60E-05 mg/mL. These antioxidant activities of the extract may be due to the presence of polyphenols and especially flavonoids and tannins.

Estimation du contenu phenolique et evaluation de l’activite antioxydante de l’extrait hydromethanolique de teucrium flavum l. [document électronique] / Hadil Doumi, Auteur ; Taklit Ferhoune, Auteur ; Hassiba Benabdallah, Directeur de thèse . - Sétif : Faculté des Sciences de la Nature et de la Vie, 2024 . - 44 Feuilles.
ISSN : MS/2043
Langues : Français

Catégories :	Thèse et mémoire:Master:Physiologie cellulaire et physiopathologie
Mots-clés :	Teucrium flavum L.  Antioxidant activity  Phenolic compounds  BHT  Phenanthroline.
Résumé :	Teucrium flavum L., known by the common name of "Germandrée jaune", is a medicinal plant of the Lamiaceaes family , widely used in traditional medicine as antipyretic and healing. In this work, a hydromethanolic extract was prepared from the aerial part of this plant. The extraction yield was 24.54%. Quantitative estimation of phenolic compounds by colorimetric method showed that the extract contains 203.00 ± 0.003 mg gallic acid equivalent/g of extract, 12.00 ± 0.001 mg quercetin equivalent/g of extract and 226.00 ± 0.0002 mg tannic acid equivalent/g of extract. The evaluation of antioxidant power was performed using different tests in the presence of standard antioxidants. The results revealed that the extract showed low activity against the DPPH radical with an IC50 value of 0.0184 ± 0.0004 mg/mL compared to the standard antioxidant, gallic acid, (IC50= 0.001 ± 4.11E-05 mg/mL). For the H2O2 test, the IC50 value is 0.363 ± 0.087 mg/mL. The reducing power of the hydromethanolic extract of Teucrium flavum L. (A0.5 = 0.024 ± 0.0001 mg/mL) is lower than that of the standard antioxidant (A0.5 = 0.0143 ± 0.0003 mg/mL). Same thing was observed for the phenanthroline assay where the extract showed low activity (A0.5 = 0.012 ± 0.001 mg/mL) compared to BHT whose A0.5 value was 0.002 ± 7.60E-05 mg/mL. These antioxidant activities of the extract may be due to the presence of polyphenols and especially flavonoids and tannins.