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Résumé :
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The purpose of this research was to assess how pure flavonoids (Catechin, Fisetin, Rutin, and Morin) affect inflammation and oxidative stress, particularly in terms of their impact on AAPH-induced hemolysis, Myeloperoxidase, and Elastase activities. The study evaluated the antioxidant properties of these flavonoids in vitro and found they provided substantial protection against AAPH-induced red blood cell hemolysis. There was a clear relationship observed between the concentration of flavonoids and the time taken to achieve half-hemolysis (HT50), indicating their potential as antioxidants. The observed half-hemolysis time (HT50) values in the anti-hemolytic assay were as follows: for catechin at 5 and 1 μg/ml, the HT50 values were 4.22 h and 3.83 h, respectively, indicating significant effects. Fisetin showed HT50 values of 3.58 h and 1.98 h at 5 and 1 μg/ml, respectively. Rutin exhibited HT50 values of 2.95 h and non -significant effect of 1.65 h at 5 and 1 μg/ml, respectively. Morin demonstrated non-significant effects with HT50 values of 1.92 h and 2.04 h. The research on flavonoids also revealed notable inhibition of elastase and MPO activities. The anti-inflammatory properties were validated by assessing how flavonoids inhibit both elastase and neutrophil myeloperoxidase activities. The flavonoids gave the following inhibitory effects on myeloperoxidase activity: morin and catechin demonstrated high inhibition rates of 67.26% and 70.33% at 5 μg/ml, and 38.47% and 39.92% at 1 μg/ml.followed by Fisetin and Rutin with lower effect (40,34% and 29,23% at 5μg/ml, and 41,38% and ,22.14% at 1μg/ml).The effect on elastase activity was as following: Catechin had significant effect at both concentrations 10μg/ml and 5μg/ml with inhibition percentage of 18,08% and 11,42% respectively. Fisetin also had significant effect at both concentrations with 19,02% at 10 μg/ml and 13,72 % at 5μg/ml. Morin had non-significant effect whith both concentrations (0,69% and 1,10 %). Rutin showed a moderated effect at concentration 1 μg/ml with 5,27% inhibition, and non-significant inhibition 3.32% at concentration 5μg/ml.The results obtained indicate that flavonoids exhibit significant effects in reducing hemolysis, acting as antioxidants, and displaying anti-inflammatory properties.
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