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| Titre : | Phytochemical profiling, antioxidant, and anti-inflammatory activities of Moringa oleifera Lam. : an ethnopharmacological and experimental study | | Type de document : | document électronique | | Auteurs : | Ouail Imad Eddine Bouharizi, Auteur ; Mostefa Derouiche, Auteur ; Karbab,Ahlem, Directeur de thèse | | Editeur : | Faculté des sciences de la nature et de la vie | | Année de publication : | 2025 | | Importance : | 24 Feuilles | | ISBN/ISSN/EAN : | MS/2204 | | Langues : | Français | | Catégories : | Thèse et mémoire:Master:Biochimie appliquée
| | Mots-clés : | Moringa oleifera Lam. Ethnopharmacological study Antioxidant activity Anti- inflammatory activity Polyphenols. | | Résumé : |
Moringa oleifera Lam. is a medicinal plant used in traditional medicine to cure various ailments. This study aimed to evaluate the ethnopharmacological study, antioxidant and anti-inflammatory activities. An ethnopharmacological survey was conducted to gather detailed information regarding the traditional usage (55.4%) of different plant parts, diseases treated, and routes of administration. In addition to traditional knowledge documentation, phytochemical analyses of a dried extract (DE) of M. oleifera leaves were performed. Quantification of secondary metabolites revealed high levels of polyphenols, flavonoids and condensed tannins with a values of
255.79 ± 6.82 μg gallic acid equivalent, 4.64 ± 0.52 μg quercetin equivalent, and 41.47 ± 1.41 μg catechin equivalent dry extract, respectively. In antioxidant activity using the DPPH radical scavenging assay, the results demonstrated a potent free radical inhibition with an IC₅₀ of
0.15 ± 0.01 mg/mL. In vitro assays measuring the inhibition of protein denaturation revealed dose- dependent anti-inflammatory activity of the extract, achieving 82.57 ± 0.00% inhibition at 8 mg/mL. In vivo study using xylene-induced ear edema in mice showed a significant inhibition of inflammation at a dose of 2 mg/mL, with inhibition percentages reaching 87.65%. Finally, the findings of the acute toxicity experiment demonstrated that extract is safe and that even at dosages as high as 2 g/kg DW. These findings provide strong scientific support for the traditional uses of Moringa oleifera and highlight its promising potential as a source of natural antioxidants and anti- inflammatory agents. Further investigation is warranted to isolate and characterize the specific active compounds responsible for these effects. |
Phytochemical profiling, antioxidant, and anti-inflammatory activities of Moringa oleifera Lam. : an ethnopharmacological and experimental study [document électronique] / Ouail Imad Eddine Bouharizi, Auteur ; Mostefa Derouiche, Auteur ; Karbab,Ahlem, Directeur de thèse . - Setif, Algerie : Faculté des sciences de la nature et de la vie, 2025 . - 24 Feuilles. ISSN : MS/2204 Langues : Français | Catégories : | Thèse et mémoire:Master:Biochimie appliquée
| | Mots-clés : | Moringa oleifera Lam. Ethnopharmacological study Antioxidant activity Anti- inflammatory activity Polyphenols. | | Résumé : |
Moringa oleifera Lam. is a medicinal plant used in traditional medicine to cure various ailments. This study aimed to evaluate the ethnopharmacological study, antioxidant and anti-inflammatory activities. An ethnopharmacological survey was conducted to gather detailed information regarding the traditional usage (55.4%) of different plant parts, diseases treated, and routes of administration. In addition to traditional knowledge documentation, phytochemical analyses of a dried extract (DE) of M. oleifera leaves were performed. Quantification of secondary metabolites revealed high levels of polyphenols, flavonoids and condensed tannins with a values of
255.79 ± 6.82 μg gallic acid equivalent, 4.64 ± 0.52 μg quercetin equivalent, and 41.47 ± 1.41 μg catechin equivalent dry extract, respectively. In antioxidant activity using the DPPH radical scavenging assay, the results demonstrated a potent free radical inhibition with an IC₅₀ of
0.15 ± 0.01 mg/mL. In vitro assays measuring the inhibition of protein denaturation revealed dose- dependent anti-inflammatory activity of the extract, achieving 82.57 ± 0.00% inhibition at 8 mg/mL. In vivo study using xylene-induced ear edema in mice showed a significant inhibition of inflammation at a dose of 2 mg/mL, with inhibition percentages reaching 87.65%. Finally, the findings of the acute toxicity experiment demonstrated that extract is safe and that even at dosages as high as 2 g/kg DW. These findings provide strong scientific support for the traditional uses of Moringa oleifera and highlight its promising potential as a source of natural antioxidants and anti- inflammatory agents. Further investigation is warranted to isolate and characterize the specific active compounds responsible for these effects. |
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Phytochemical profiling, antioxidant, and anti-inflammatory activities of Moringa oleifera Lam. / Faculté des sciences de la nature et de la vie (2025)
