Catalogue de la bibliothèque de SNV
| Titre : | Assessment of biological activities of Marrubium sp hydroethanolic extract |
| Auteurs : | Yasmine Bara, Auteur ; Mohamed Khaled Belatel, Auteur ; Hibat allah Benmeziane, Auteur ; Walid Mamache, Directeur de thèse |
| Type de document : | document électronique |
| Editeur : | Sétif (Algérie) : Faculté des sciences de la Nature et de la vie, Université Ferhat Abbas Sétif 1, 2025 |
| ISBN/ISSN/EAN : | MS/2283 |
| Format : | 31 Feuilles / PDF |
| Langues: | Anglais |
| Catégories : | |
| Mots-clés: | Marrubium sp ; Antioxidant activity ; Gastric emptying ; Intestinal transit and acute toxicity. |
| Résumé : |
The present study investigated the phytochemical content, antioxidant activity, toxicological effects of Marrubium hydroethanolic extract (MHE), and its impact on gastric emptying (GE) and intestinal transit (IT) rates. The extraction yield was 21.66%. Quantitative analysis revealed high levels of bioactive compounds, including total polyphenols (95 ±1.010 GAE μg/mg), flavonoids (54.43 ± 11.37 QE μg/mg), and tannins (77.06 ± 0.47 TAE μg/mg). MHE exhibited notable antioxidant properties across multiple assays: DPPH radical scavenging (IC₅₀ = 98.86 ± 2.09 μg/mL), ABTS assay (IC₅₀ = 24.40 ± 1.24 μg/mL), ferrozine-based iron chelation (IC₅₀ = 210.71 ± 1.59 μg/mL), and phenanthroline-based reducing power (EC₅₀ = 560.18 ± 20.47 μg/mL). Acute toxicity studies revealed no mortality or behavioral alterations, even at high doses (up to 5 g/kg). Biochemical assays showed a significant reduction in hepatic enzymes (ALP, AST, ALT), while creatinine levels remained stable, suggesting hepatoprotective activity without nephrotoxicity. At the 5 g/kg dose, oxidative stress biomarkers showed improved antioxidant defense: glutathione (GSH) levels were 2.10 ± 0.24 μmol TNB/g in kidneys and 4.06 ± 0.82 μmol TNB/g in liver tissue; malondialdehyde (MDA) levels were 43.6 nmol/g in kidney tissue and 34.87 nmol/g in liver. Catalase (CAT) activity reached 5.85 ± 0.33 mmol/min/mg (kidney) and 6.86 ± 4.1 mmol/min/mg (liver), while total protein content was 4.56 ± 0.26 mg/mL in kidneys and 6.24 ± 0.55 mg/mL in liver. Gastric emptying and Intestinal transit evaluations showed that MHE at 100 mg/kg significantly inhibited gastric emptying (32.9%), whereas the 50 mg/kg dose showed no significant difference from the control (24.4%). Both doses exhibited similar significant inhibition effects on intestinal transit (13-14%). These findings support the potential therapeutic use of MHE as an antioxidant and hepatoprotective agent with gastrointestinal inhibition effects. |
Exemplaires
| Code-barres | Cote | Support | Localisation | Section | Disponibilité |
|---|---|---|---|---|---|
| MS/2283 | BAPP/CD269 | DVD et CD | Bibliothèque SNV | Englais | Disponible |
