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| Titre : | Phytochemical Analysis, Antioxidant Capacity, and Biological Evaluation of the Aqueous Extract of Marrubium Sp |
| Auteurs : | Asma Boulahia, Auteur ; Nihal Boudiaf, Auteur ; Maroua Karoun, Auteur ; Walid Mamache, Directeur de thèse |
| Type de document : | document électronique |
| Editeur : | Sétif (Algérie) : Faculté des sciences de la Nature et de la vie, Université Ferhat Abbas Sétif 1, 2025 |
| ISBN/ISSN/EAN : | MS/2276 |
| Format : | 27 Feuilles / PDF |
| Langues: | Anglais |
| Catégories : | |
| Mots-clés: | Marribiums sp ; Aqueous extract ; Antioxidant activity ; Acute toxicity ; intestinal motility. |
| Résumé : |
The present study investigates the phytochemical composition, antioxidant activity, and biological effects of the aqueous extract from the aerial parts of Marrubium sp (MaqE). The extraction yielded a dark brown powder with a yield of 8.5%. Phytochemical screening revealed high levels of polyphenols (65.6 mg EGA/g dry weight of extract), tannins (60.46 mg ETA/g dry weight of extract), and flavonoids (3.72 mg EQ/g dry weight of extract). Antioxidant potential was evaluated through various in vitro assays, including DPPH, ABTS, metal chelation, and reducing power tests. The extract showed high antioxidant activity, with IC₅₀ values of 86.62 μg/mL (DPPH) and 26.27 μg/mL (ABTS), The extract also demonstrated ferrous ion chelating activity (IC₅₀ = 38.97 ± 1.28 μg/mL) and reducing power (A₀.₅ = 399.01 ± 18.33), these values were significantly lower than standards. In vivo, in acute toxicity, rats treated orally for 15 days with 2 and 5 g/kg doses of the extract showed no significant changes in body or organ weights. Serum biochemical parameters revealed significant decreases in ALP (143.67 ± 12.7 and 100.67 ± 5.78 IU/L), ALT (82.41 ± 22.12 and 80.66 ± 13.37 IU /L), and a non-significant decrease in AST. Serum creatinine levels remained unchanged, confirming no nephrotoxicity. Oxidative stress markers in liver and kidney tissues showed no significant changes in MDA levels or CAT activity. However, liver GSH levels significantly decreased (2.69 ± 0.67 and 2.13 ± 0.20 μmol/g), accompanied by a significant reduction in total protein content (7.23 ± 0.34 and 4.66 ± 0.53 mg/ml; respectively). Kidney GSH and total protein levels showed no significant differences. Liver and kidney catalase and MDA levels remained stable, though total protein and GSH levels in liver tissues decreased at higher doses. Additionally, the extract inhibited gastrointestinal motility in mice, reducing IT in a dose-dependent manner with 71.38% ± 1.56 at a dose of 50 mg/kg and 69.68% ± 3.55 at a dose of 100 mg/kg. As for gastric emptying, a significant decrease of 33.39% ± 23.85 was recorded at the 100 mg/kg dose, which is higher than that observed with atropine, suggesting potential antispasmodic activity. In conclusion, the MAqE exhibits promising antioxidant and gastrointestinal regulatory properties, with a favorable safety profile. These results support its traditional medicinal use and highlight its potential for further development in therapeutic applications. |
Exemplaires
| Code-barres | Cote | Support | Localisation | Section | Disponibilité |
|---|---|---|---|---|---|
| MS/2276 | BAPP/CD262 | DVD et CD | Bibliothèque SNV | Englais | Disponible |
