Titre :	Antioxidant and immunomodulatory proprieties of Papaver rhoeas
Type de document :	document électronique
Auteurs :	Rahma Sacia Laouachria, Auteur ; Hind Rachedi, Auteur ; Nacer Amraoui, Directeur de thèse
Editeur :	Sétif [Algérie] : Faculté des sciences de la Nature et de la vie, Université Ferhat Abbas Sétif 1
Année de publication :	2025
Importance :	27 Feuilles
Format :	PDF
ISBN/ISSN/EAN :	MS/2263
Langues :	Anglais
Catégories :	Thèse et mémoire:Master:Immunologie
Mots-clés :	Papaver rhoeas  DPPH  Immunomodulatory  Polyphenols  Flavonoids.
Résumé :	Papaver rhoeas belongs to the family of “Papaveraceae”. It was used since ancient times for its healing properties. Our study aims to evaluate the antioxidant and immunomodulatory activities of P. rhoeas hydromethanolic extract. The determination of total polyphenols and flavonoids contents were carried out using colorimetric methods, using Folin-Ciocalteu and aluminum chloride (AlCl₃), respectively. Antioxidant propriety was tested using free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), with BHT as a standard. The effect of P. rhoeas extract on the humoral immune response was evaluated in vivo in mice injected with sheep red blood cells (SRBC). The total phenolic and flavonoid contents of P. rhoeas extract showed 79.64 ± 5.09 μg GAE/mg and 11.07 ± 0.52 μg QE/mg of dry weight of the plant, respectively. In vitro antioxidant assay revealed that P. rhoeas exhibited a strong antioxidant activity (IC50 = 104.33 ± 3.05 μg/ml) compared to BHT. The immunomodulatory activity showed that the treatment with P. rhoeas extract at the dose of 200 mg/kg demonstrated a significant increase in antibody titre (210.44 ± 24.38), while at the dose of 400 mg/kg showed a decrease in this titre (107.55 ± 13.38). In conclusion, the hydromethanolic extract of P. rhoeas has potent antioxidant and immunomodulatory activities (immunostimulants at low dose, and immunosuppressants at high dose).

Antioxidant and immunomodulatory proprieties of Papaver rhoeas [document électronique] / Rahma Sacia Laouachria, Auteur ; Hind Rachedi, Auteur ; Nacer Amraoui, Directeur de thèse . - Sétif (Algérie) : Faculté des sciences de la Nature et de la vie, Université Ferhat Abbas Sétif 1, 2025 . - 27 Feuilles ; PDF.
ISSN : MS/2263
Langues : Anglais

Catégories :	Thèse et mémoire:Master:Immunologie
Mots-clés :	Papaver rhoeas  DPPH  Immunomodulatory  Polyphenols  Flavonoids.
Résumé :	Papaver rhoeas belongs to the family of “Papaveraceae”. It was used since ancient times for its healing properties. Our study aims to evaluate the antioxidant and immunomodulatory activities of P. rhoeas hydromethanolic extract. The determination of total polyphenols and flavonoids contents were carried out using colorimetric methods, using Folin-Ciocalteu and aluminum chloride (AlCl₃), respectively. Antioxidant propriety was tested using free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), with BHT as a standard. The effect of P. rhoeas extract on the humoral immune response was evaluated in vivo in mice injected with sheep red blood cells (SRBC). The total phenolic and flavonoid contents of P. rhoeas extract showed 79.64 ± 5.09 μg GAE/mg and 11.07 ± 0.52 μg QE/mg of dry weight of the plant, respectively. In vitro antioxidant assay revealed that P. rhoeas exhibited a strong antioxidant activity (IC50 = 104.33 ± 3.05 μg/ml) compared to BHT. The immunomodulatory activity showed that the treatment with P. rhoeas extract at the dose of 200 mg/kg demonstrated a significant increase in antibody titre (210.44 ± 24.38), while at the dose of 400 mg/kg showed a decrease in this titre (107.55 ± 13.38). In conclusion, the hydromethanolic extract of P. rhoeas has potent antioxidant and immunomodulatory activities (immunostimulants at low dose, and immunosuppressants at high dose).