Catalogue de la bibliothèque de SNV
| Titre : | Affinity chromatography : methods and protocols |
| Auteurs : | Michael Zachariou, Editeur scientifique |
| Type de document : | texte imprimé |
| Mention d'édition : | Second ed. |
| Editeur : | [Totowa (N.J.)] : Humana press, 2008 |
| Collection : | Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, num. 421 |
| ISBN/ISSN/EAN : | 978-1-59745-582-4 |
| Format : | 1 vol. (XII-343 p.) / ill. / 24 cm |
| Langues: | Anglais |
| Index. décimale : | 543.8 (Chromatographie) |
| Catégories : |
Biochimie - Biotechnologie - Génie chimique - Génétique ... Chimie ( Organique -Générale- Théorique - Analytique -Quantique - Géochimie - Physique ) |
| Mots-clés: | - Chromatographie - Chromatographie d'affinité |
| Résumé : |
This chapter covers the use of immobilized metal ion affinity chromatography (IMAC) for enrichment of phosphorylated proteins. Some requirements for successful enrichment of these types of proteins are discussed. An experimental protocol and a set of application data are included to enable the scientist to obtain high-yield results in a very short time with pre-packed phospho-specific metal ion affinity resin (PMAC). |
| Note de contenu : |
Sommaire: Roque, Ana Cecília A. (et al.)Pages 1-23 Part 1: Various modes of affinity Chromatography Immobilized Metal Ion Affinity Chromatography of Native Proteins Charlton, Adam (et al.Pages 25-36 Affinity Precipitation of Proteins Using Metal Chelates Kumar, Ashok (et al.)Pages 37-52 Immunoaffinity Chromatography Gallant, Stuart R. (et al.)Pages 53-60 Dye Ligand Chromatography allant, Stuart R. (et al.)Pages 61-70 Purification of Proteins Using Displacement Chromatography Tugcu, NihalPages 71-90 Part 2:Affinity Chromatography using Rationally Designed Ligands for Use in Affinity Chromatography Roque, Ana Cecília A. (et al.)Pages 93-110 Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography Casey, Joanne L. (et al.)Pages 111-124 Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein Forde, Gareth M.Pages 125-136 Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins Charlton, Adam (et al.)Pages 137-150 Methods for the Purification of HQ-Tagged Proteins Godat, Becky (et al.)Pages 151-168 Amylose Affinity Chromatography of Maltose-Binding Protein Pattenden, Leonard K (et al.)Pages 169-190 Methods for Detection of Protein–Proteinnl and Protein–DNA Interactions Using HaloTag ™ Urh, Marjeta (et al.)Pages 191-210 Site-Specific Cleavage of Fusion Proteins Charlton, Adam Pages 211-228 The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags Arnau, José (et al.)Pages 229-243 Part 3:Various applications of Affinity Chromatography Affinity Processing of Cell-Containing Feeds Using monolithic Macroporous Hydrogels, Cryogels Galaev, Igor Yu. (et al.)Pages 247-256 Monolithic Bioreactors for Macromolecules Benčina, Mojca (et al.)Pages 257-274 Plasmid DNA Purification Via the Use of a Dual Affinity Protein Forde, Gareth M.Pages 275-284 Affinity Chromatography of Phosphorylated Proteins Tchaga, Grigoriy S.Pages 285-294 Protein Separation Using Immobilized Phospholipid Chromatography Lee, Tzong-Hsien (et al.)Pages 295-302 Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis Heegaard, Niels H. H. (et al.) |
Exemplaires
| Code-barres | Cote | Support | Localisation | Section | Disponibilité |
|---|---|---|---|---|---|
| FB/06653 | SNV8/0933 | Livre | Bibliothèque SNV | Englais | Disponible |
| FB/06654 | SNV8/0933 | Livre | Bibliothèque SNV | Englais | Disponible |
| FB/06655 | SNV8/0933 | Livre | Bibliothèque SNV | Englais | Disponible |
| FB/06656 | SNV8/0933 | Livre | Bibliothèque SNV | Englais | Disponible |
| FB/06680 | SNV8/0933 | Livre | Bibliothèque SNV | Englais | Disponible |
| FB/06681 | SNV8/0933 | Livre | Bibliothèque SNV | Englais | Disponible |
