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| Titre : | Affinity chromatography : methods and protocols | | Type de document : | texte imprimé | | Auteurs : | Michael Zachariou, Editeur scientifique | | Mention d'édition : | Second ed. | | Editeur : | [Totowa (N.J.)] : Humana press | | Année de publication : | 2008 | | Collection : | Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745 num. 421 | | Importance : | 1 vol. (XII-343 p.) | | Présentation : | ill. | | Format : | 24 cm | | ISBN/ISSN/EAN : | 978-1-59745-582-4 | | Langues : | Anglais | | Catégories : | Biochimie - Biotechnologie - Génie chimique - Génétique ...
Chimie ( Organique -Générale- Théorique - Analytique -Quantique - Géochimie - Physique )
| | Mots-clés : | - Chromatographie
- Chromatographie d'affinité | | Index. décimale : | 543.8 Chromatographie | | Résumé : |
This chapter covers the use of immobilized metal ion affinity chromatography (IMAC) for enrichment of phosphorylated proteins. Some requirements for successful enrichment of these types of proteins are discussed. An experimental protocol and a set of application data are included to enable the scientist to obtain high-yield results in a very short time with pre-packed phospho-specific metal ion affinity resin (PMAC). | | Note de contenu : |
Sommaire:
Roque, Ana Cecília A. (et al.)Pages 1-23
Part 1: Various modes of affinity Chromatography
Immobilized Metal Ion Affinity Chromatography of Native Proteins
Charlton, Adam (et al.Pages 25-36
Affinity Precipitation of Proteins Using Metal Chelates
Kumar, Ashok (et al.)Pages 37-52
Immunoaffinity Chromatography
Gallant, Stuart R. (et al.)Pages 53-60
Dye Ligand Chromatography
allant, Stuart R. (et al.)Pages 61-70
Purification of Proteins Using Displacement Chromatography
Tugcu, NihalPages 71-90
Part 2:Affinity Chromatography using
Rationally Designed Ligands for Use in Affinity Chromatography
Roque, Ana Cecília A. (et al.)Pages 93-110
Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography
Casey, Joanne L. (et al.)Pages 111-124
Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein
Forde, Gareth M.Pages 125-136
Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins
Charlton, Adam (et al.)Pages 137-150
Methods for the Purification of HQ-Tagged Proteins
Godat, Becky (et al.)Pages 151-168
Amylose Affinity Chromatography of Maltose-Binding Protein
Pattenden, Leonard K (et al.)Pages 169-190
Methods for Detection of Protein–Proteinnl and Protein–DNA Interactions Using HaloTag ™
Urh, Marjeta (et al.)Pages 191-210
Site-Specific Cleavage of Fusion Proteins
Charlton, Adam Pages 211-228
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
Arnau, José (et al.)Pages 229-243
Part 3:Various applications of Affinity Chromatography
Affinity Processing of Cell-Containing Feeds Using monolithic Macroporous Hydrogels, Cryogels
Galaev, Igor Yu. (et al.)Pages 247-256
Monolithic Bioreactors for Macromolecules
Benčina, Mojca (et al.)Pages 257-274
Plasmid DNA Purification Via the Use of a Dual Affinity Protein
Forde, Gareth M.Pages 275-284
Affinity Chromatography of Phosphorylated Proteins
Tchaga, Grigoriy S.Pages 285-294
Protein Separation Using Immobilized Phospholipid Chromatography
Lee, Tzong-Hsien (et al.)Pages 295-302
Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis
Heegaard, Niels H. H. (et al.) |
Affinity chromatography : methods and protocols [texte imprimé] / Michael Zachariou, Editeur scientifique . - Second ed. . - [Totowa (N.J.)] : Humana press, 2008 . - 1 vol. (XII-343 p.) : ill. ; 24 cm. - ( Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745; 421) . ISBN : 978-1-59745-582-4 Langues : Anglais | Catégories : | Biochimie - Biotechnologie - Génie chimique - Génétique ...
Chimie ( Organique -Générale- Théorique - Analytique -Quantique - Géochimie - Physique )
| | Mots-clés : | - Chromatographie
- Chromatographie d'affinité | | Index. décimale : | 543.8 Chromatographie | | Résumé : |
This chapter covers the use of immobilized metal ion affinity chromatography (IMAC) for enrichment of phosphorylated proteins. Some requirements for successful enrichment of these types of proteins are discussed. An experimental protocol and a set of application data are included to enable the scientist to obtain high-yield results in a very short time with pre-packed phospho-specific metal ion affinity resin (PMAC). | | Note de contenu : |
Sommaire:
Roque, Ana Cecília A. (et al.)Pages 1-23
Part 1: Various modes of affinity Chromatography
Immobilized Metal Ion Affinity Chromatography of Native Proteins
Charlton, Adam (et al.Pages 25-36
Affinity Precipitation of Proteins Using Metal Chelates
Kumar, Ashok (et al.)Pages 37-52
Immunoaffinity Chromatography
Gallant, Stuart R. (et al.)Pages 53-60
Dye Ligand Chromatography
allant, Stuart R. (et al.)Pages 61-70
Purification of Proteins Using Displacement Chromatography
Tugcu, NihalPages 71-90
Part 2:Affinity Chromatography using
Rationally Designed Ligands for Use in Affinity Chromatography
Roque, Ana Cecília A. (et al.)Pages 93-110
Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography
Casey, Joanne L. (et al.)Pages 111-124
Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein
Forde, Gareth M.Pages 125-136
Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins
Charlton, Adam (et al.)Pages 137-150
Methods for the Purification of HQ-Tagged Proteins
Godat, Becky (et al.)Pages 151-168
Amylose Affinity Chromatography of Maltose-Binding Protein
Pattenden, Leonard K (et al.)Pages 169-190
Methods for Detection of Protein–Proteinnl and Protein–DNA Interactions Using HaloTag ™
Urh, Marjeta (et al.)Pages 191-210
Site-Specific Cleavage of Fusion Proteins
Charlton, Adam Pages 211-228
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
Arnau, José (et al.)Pages 229-243
Part 3:Various applications of Affinity Chromatography
Affinity Processing of Cell-Containing Feeds Using monolithic Macroporous Hydrogels, Cryogels
Galaev, Igor Yu. (et al.)Pages 247-256
Monolithic Bioreactors for Macromolecules
Benčina, Mojca (et al.)Pages 257-274
Plasmid DNA Purification Via the Use of a Dual Affinity Protein
Forde, Gareth M.Pages 275-284
Affinity Chromatography of Phosphorylated Proteins
Tchaga, Grigoriy S.Pages 285-294
Protein Separation Using Immobilized Phospholipid Chromatography
Lee, Tzong-Hsien (et al.)Pages 295-302
Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis
Heegaard, Niels H. H. (et al.) |
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