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Titre : | Extraction and preparation of natural biologically active molecules for pharmaceutical uses |
Auteurs : | Imane Touati, Auteur ; Souad Zehri, Auteur ; Saliha Dahamna, Directeur de thèse |
Type de document : | document électronique |
Editeur : | Sétif : Faculté des Sciences de la Nature et de la Vie, 2023 |
ISBN/ISSN/EAN : | MS/1636 |
Langues: | Anglais |
Catégories : | |
Mots-clés: | Medicinal plants ; Active substances ; Extraction ; Caffeine ; Catechin. |
Résumé : |
Plants have an interestingly high medicinal and nutritional value, due to the biologically active substances they contain, which explains why the world has resorted to using them in the manufacture and formulation of various medicines and nutritional supplements. Our research deals with a general study of the active substances found in medicinal plants and their extraction methods. Firstly, we got acquainted with the medicinal plants, secondly, the secondary metabolites (alkaloids, glycosides, terpenes, flavonoids, etc.), and thirdly, the methods of extracting these molecules. In this work, two medicinal plants, coffee (coffea) and tea (Camellia sinensis), were studied and caffeine and catechin were identified. In this study, the active substance caffeine was extracted from 50 grams of coffee using the dichloromethane solvent and the liquid-solid and liquid-liquid extraction methods, and its purity was confirmed by a spectrophotometry and compared with the pure caffeine standard. The quantitative evaluation of caffeine was also studied and compared with theoretical references. On the other side, catechin was extracted from 20 grams of green tea using the same method and the solvent used was ethyl acetate. Its identity was confirmed using the same device and compared to the catechin standard, as was the quantitative evaluation of catechin compared with theoretical references. According to the results obtained, the amount of pure caffeine obtained was estimated at 1% (a relatively small amount), while the amount of catechin was estimated at 2.15% (a moderate amount), and the results of the study also allowed us to confirm the purity of both compounds. |
Exemplaires
Code-barres | Cote | Support | Localisation | Section | Disponibilité |
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MS/1636 | PCP/CD162 | DVD et CD | Bibliothèque SNV | Englais | Disponible |